Differentiation of Microorganisms Lab Report Example | Topics and Well Written Essays - 1000 words

Differentiation of Microorganisms - Lab Report compositors caseThe researcher tells that in burnish media experiment the test included a artificial medium, tryptic soy broth, SF broth, Macconkey Agar, and alimental agar. The liquid media like the nutrient broth was use in the reproduction and isolation of organisms. McConkey Agar was a solid medium that examined colony morphology and the isolation of the organisms. Most of the culture media were complex their chemical motif was unknown. Additionally, the culture media were postulateive. They selected certain microbe types by supplying the growth conditions. An example of this media was SF medium it was designed to select Enterococcus faecallis in warm-blooded animals. In Enzymes experiment, the tests included the Caseinase which hydrolyzed casein to peptides to amino acids, gelatinase hydrolyzed gelatin, and Catalase protected the cells from the hydrogen peroxide. In addition, Oxidase indicated the carriage of cytochrome in th e microorganisms, during oxidation the compounds turned red or blue. In fermentation experiment, the fermentable compounds were sugar (glucose, sucrose, and lactose). Consequently, an power dye was used to change the color from red to a yellow color when the PH is down the stairs 7. In motility, experiment flagellum was used a bacteriuml motility. The experiment used several ship canal to determine motility i.e. hanging drop and motility agar. In the course of the experiment, if the culture tested is harsh aerobe, then growth will occur on the tube surface. The hanging drop slide was used in motility viewing when the oxygen is depleted.In the experiment that dealt with differentiation of enteric bacteria, most bacteria used were facultative anaerobes and anaerobes. The enteric bacteria used here encompassed facultative anaerobic rods, gram negative, and non-spore forming bacteria. The Coliforms were characterized by their fermenting ability. Other tests that were used to identify the enteric bacteria included H2S, KCN, MB agar, TSI agar, SS agar, and MacConkey agar. Discussion The culture media experiment used the following microorganism Escherichis Coli, Enterococcus faecalis, and Pseudomonas aeruginosa. The two culture media in the experiment were synthetic medium and tryptic Soy Broth. The